Citation:
Albeanu DF, Soucy E, Sato TF, Meister M, Murthy VN. LED arrays as cost effective and efficient light sources for widefield microscopy. PLoS One [Internet]. 2008;3:e2146.
Abstract:
New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.
Notes:
Albeanu, Dinu FSoucy, EdwardSato, Tomokazu FMeister, MarkusMurthy, Venkatesh NResearch Support, Non-U.S. Gov'tUnited StatesPloS onePLoS One. 2008 May 14;3(5):e2146.
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