An Integrated Microfluidic Probe for Concentration-Enhanced Selective Single Cell Kinase Activity Measurement

Citation:

Sarkar A, Kolitz S, Cheow LF, Lauffenburger DA, Han J. An Integrated Microfluidic Probe for Concentration-Enhanced Selective Single Cell Kinase Activity Measurement. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2011) [Internet]. 2011 :1394-1396.

Abstract:

We present an integrated microfluidic probe that captures the contents of selected single cells from a cell population adhe-rent on a standard tissue culture platform and directly measures specific protein kinase activities in the captured single cell ly-sate. A kinetic fluorimetric kinase assay in a small integrated chamber isolated by micro-valves and an end-point concentra-tion-enhanced mobility-shift kinase assay in an integrated nanofluidic concentrator are demonstrated. We demonstrate the use of the probe by measuring MAPKAPK2 and AKT kinase activity in single human hepatocellular carcinoma (HepG2) cells.

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Last updated on 03/11/2014