Characterizing ABC-Transporter Substrate-Likeness Using a Clean-Slate Genetic Background

Citation:

Artem Sokolov, Stephanie Ashenden, Nil Sahin, Richard Lewis, Nurdan Erdem, Elif Ozaltan, Andreas Bender, Frederick P Roth, and Murat Cokol. 2019. “Characterizing ABC-Transporter Substrate-Likeness Using a Clean-Slate Genetic Background.” Front Pharmacol, 10, Pp. 448.

Abstract:

Mutations in ATP Binding Cassette (ABC)-transporter genes can have major effects on the bioavailability and toxicity of the drugs that are ABC-transporter substrates. Consequently, methods to predict if a drug is an ABC-transporter substrate are useful for drug development. Such methods traditionally relied on literature curated collections of ABC-transporter dependent membrane transfer assays. Here, we used a single large-scale dataset of 376 drugs with relative efficacy on an engineered yeast strain with all ABC-transporter genes deleted (ABC-16), to explore the relationship between a drug's chemical structure and ABC-transporter substrate-likeness. We represented a drug's chemical structure by an array of substructure keys and explored several machine learning methods to predict the drug's efficacy in an ABC-16 yeast strain. Gradient-Boosted Random Forest models outperformed all other methods with an AUC of 0.723. We prospectively validated the model using new experimental data and found significant agreement with predictions. Our analysis expands the previously reported chemical substructures associated with ABC-transporter substrates and provides an alternative means to investigate ABC-transporter substrate-likeness.