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Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale

Citation:

Xia, B. ; Han, D. ; Lu, X. ; Sun, Z. ; Zhou, A. ; Yin, Q. ; Zeng, H. ; Liu, M. ; Jiang, X. ; Xie, W. ; et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale. Nat Methods 2015, 12, 1047-50.

Date Published:

2015 Nov

Abstract:

Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.

Last updated on 04/17/2024