@article {691994,
	title = {Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale},
	journal = {Nat Methods},
	volume = {12},
	number = {11},
	year = {2015},
	month = {2015 Nov},
	pages = {1047-50},
	abstract = {Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.},
	keywords = {5-Methylcytosine, Animals, Cell Line, CpG Islands, Cytosine, DNA Methylation, DNA Primers, Epigenomics, Gene Expression Regulation, Genome, Mice, Mice, Transgenic, Oligonucleotides, Oxygen, Polymerase Chain Reaction, Sequence Analysis, DNA, Stem Cells, Sulfites},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3569},
	author = {Bo Xia and Han, Dali and Lu, Xingyu and Sun, Zhaozhu and Zhou, Ankun and Yin, Qiangzong and Zeng, Hu and Liu, Menghao and Jiang, Xiang and Xie, Wei and Chuan He and Yi, Chengqi}
}
