Zazzi-Boulou A-G, Zimkus BM, Goma-Tchimbakala J, Boudzoumou S, Mboungou-Louiki A, Jackson K. Herpetological survey of the proposed Bambama-Sanaga Mine Site, Department of Lékoumo, Republic of Congo. Herpetological Conservation and Biology 2019;14(2):470–480.
Deichmann JL, Mulcahy DG, Vanthomme H, Tobi E, Wynn AH, Zimkus BM, McDiarmid RW. How many species and under what names? Using DNA barcoding and GenBank data for west Central African amphibian conservation. [Internet]. PLoS ONE 2017;12(11):e0187283. Publisher's Version
Zimkus BM, Lawson LP, Bare MF, Barratt CD, Channing A, Dash KM, Dehling JM, Du Preez L, Gehring P-S, Greenbaum E, Gvoždík V, Harvey J, Kielgast J, Kusumba C, Nagy ZT, Pabijan M, Penner J, Rödel M-O, Vences M, Letters S. Leapfrogging into new territory: How Mascarene ridged frogs diversified across Africa and Madagascar to maintain their ecological niche. [Internet]. Molecular Phylogenetics and Evolution 2017;106(254):269. Publisher's Version
Zimkus BM, Ford LS. Best practices for genetic resources associated with natural history collections: a practical implementation. Collection Forum 2014;28(1-2):77–113.
Zimkus BM, Ford LS. Genetic resource collections associated with natural history museums: A survey and analysis to establish a benchmark of standards. DNA Banking for the 21st Century. Proceedings of the U.S. Workshop on DNA Banking. 2014;:9–44. zimkus_ford_2014.pdf
Gower DJ, Aberra RK, Schwaller S, Largen MJ, Collen B, Spawls S, Menegon M, Zimkus BM, de Sá R, Mengistu AA, others. Long-term data for endemic frog genera reveal potential conservation crisis in the Bale Mountains, Ethiopia. Oryx 2013;47:59–69.
Zimkus BM, Larson JG. Assessment of the amphibians of Bateke Plateau National Park, Gabon, including results of chytrid pathogen tests. Salamandra 2013;49:159–170.
Zimkus BM, Gvoždík V. Sky Islands of the Cameroon Volcanic Line: a diversification hot spot for puddle frogs (Phrynobatrachidae: Phrynobatrachus) [Internet]. Zoologica Scripta 2013;42:591–611. Publisher's Version
Zimkus BM, Lawson L, Loader SP, Hanken J. Terrestrialization, miniaturization and rates of diversification in African puddle frogs (Anura: Phrynobatrachidae). PLoS One 2012;7(4):e35118.Abstract
Terrestrialization, the evolution of non-aquatic oviposition, and miniaturization, the evolution of tiny adult body size, are recurring trends in amphibian evolution, but the relationships among the traits that characterize these phenomena are not well understood. Furthermore, these traits have been identified as possible "key innovations" that are predicted to increase rates of speciation in those lineages in which they evolve. We examine terrestrialization and miniaturization in sub-Saharan puddle frogs (Phrynobatrachidae) in a phylogenetic context to investigate the relationship between adaptation and diversification through time. We use relative dating techniques to ascertain if character trait shifts are associated with increased diversification rates, and we evaluate the likelihood that a single temporal event can explain the evolution of those traits. Results indicate alternate reproductive modes evolved independently in Phrynobatrachus at least seven times, including terrestrial deposition of eggs and terrestrial, non-feeding larvae. These shifts towards alternate reproductive modes are not linked to a common temporal event. Contrary to the "key innovations" hypothesis, clades that exhibit alternate reproductive modes have lower diversification rates than those that deposit eggs aquatically. Adult habitat, pedal webbing and body size have no effect on diversification rates. Though these traits putatively identified as key innovations for Phrynobatrachus do not seem to be associated with increased speciation rates, they may still provide opportunities to extend into new niches, thus increasing overall diversity.
Anthony NM, Mickala P, Abernethy KA, Atteke C, Bissiengou P, Bruford MW, Dallmeier F, Decaëns T, Dudu A, Freedman A, others. Biodiversity and conservation genetics research in Central Africa: new approaches and avenues for international collaboration. Conservation genetics resources 2012;4:523–525.
Schick S, Zimkus BM, Channing A, Köhler J, Lötters S. Systematics of ‘Little Brown Frogs’ from East Africa: recognition of Phrynobatrachus scheffleri and description of a new species from the Kakamega Forest, Kenya (Anura: Phrynobatrachidae). Salamandra 2010;46:24–36.
Zimkus BM, Schick S. Light at the end of the tunnel: insights into the molecular systematics of East African puddle frogs (Anura: Phrynobatrachidae). Systematics and Biodiversity 2010;8:39-47.
Zimkus BM, Rödel M-O, Hillers A. Complex patterns of continental speciation: molecular phylogenetics and biogeography of sub-Saharan puddle frogs (Phrynobatrachus). Molecular Phylogenetics and Evolution 2010;55(3):883-900.Abstract
Puddle frogs (Anura: Phrynobatrachidae) are one of the most species-rich sub-Saharan amphibian groups, occupying an extraordinarily diverse range of habitats. We construct the first phylogeny of puddle frogs, utilizing mitochondrial (12S rRNA, valine-tRNA, and 16S rRNA) and nuclear (RAG-1) DNA. Phylogenetic analyses are conducted using separate and combined partitions under maximum parsimony, maximum likelihood, and Bayesian criterion. Monophyly of the Phrynobatrachidae is well supported, and three major clades of Phrynobatrachus are identified. We reconstructed a biogeographic history using habitat preference, elevation, and geographic distribution. Habitat niches appear to be conserved between sister species, with the majority of species favoring forest over savanna habitats and the most recent common ancestor of the Phrynobatrachidae reconstructed as a forest species. Analyses of elevational data identify three independent colonizations of highland regions, one in each of the three major clades. Ancestral reconstructions support an East African origination of puddle frogs. Most species are restricted to one of five sub-Saharan regions and are distributed within the Eastern, Central, and Western zones with far fewer species in Southern Africa. These results elucidate the complex patterns of spatial niche partitioning that have contributed to the diversification of this widely distributed, sub-Saharan genus.