Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts


Takuma Fukunishi, Cameron A. Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K. Breuer, Jed Johnson, and Narutoshi Hibino. 2018. “Role of Bone Marrow Mononuclear Cell Seeding for Nanofiber Vascular Grafts.” Tissue engineering. Part A, 24, 1-2, Pp. 135-144.


{OBJECTIVE: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs. METHODS: Nanofiber scaffolds were fabricated from co-electrospinning a solution of polyglycolic acid and a solution of poly(i-lactide-co-e-caprolactone) and characterized with scanning electron microscopy. Platelet activation and cell seeding efficiency were assessed by ATP secretion and DNA assays, respectively. Cell-free and BM-MNC seeded scaffolds were implanted in C57BL/6 mice (n = 15/group) as infrarenal inferior vena cava (IVC) interposition conduits. Animals were followed with serial ultrasonography for 6 months, after which grafts were harvested for evaluation of patency and neotissue formation by histology and immunohistochemistry (n = 10/group) and PCR (n = 5/group) analyses. RESULTS: BM-MNC seeding of electrospun scaffolds prevented stenosis compared with unseeded scaffolds (seeded: 9/10 patent vs. unseeded: 1/10 patent