Osteoclasts...

...are the cells that clear away bone and have a characteristic actin ring and multiple nuclei (sometimes as many as 30!)
Still good...

A cell optoporated with propidium iodide (a DNA intercalator, shown in red) still managers to divide
It's a pile of FRAP!

Fluorescence Recovery After Photobleaching (FRAP) can reveal how well a protein is bound to its target in presence of a drug
Comparing extracellular matrix in disease and healthy tissue

Collagen and elastin produce a unique second harmonic signal and two photon excitation fluorescence respectively
Dead or alive

A temporary hole can be made in the cell membrane to allow proteins that normally would not enter a living cell

Clarence Yapp, DPhil (Oxon)

Platform Manager in Microscopy and Image Analysis