Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications


Morais AIS, Wang X, Vieira EG, Viana BC, Silva-Filho EC, Osajima JA, Afewerki S, Corat MAF, Silva HS, Marciano FR, Ruiz-Esparza GU, Stocco TD, Paula de MMM, and Lobo AO. 2/19/2020. “Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications.” International Journal of Nanomedicine, 2020, 15, Pp. 1173-1186. Link
Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications


Background: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications.
Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis.
Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = – 16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M.
Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.
Last updated on 07/19/2021