Joint Lecture: Optimized Experimental and Analytical Tools for Reproducible Drug-Response Studies
Caroline Shamu, Director, ICCB-Longwood Screening Facility
Caitlin Mills, Postdoctoral Fellow, Sorger Lab and Laboratory of Systems Pharmacology
Marc Hafner, Postdoctoral Fellow, Sorger Lab and Laboratory of Systems Pharmacology
Date: Tuesday, March 28
Time: 1:00-3:00 PM
Location: TMEC 227
This three-part lecture will instruct researchers in the state-of-the-art experimental and analytical tools available for conducting reproducible studies of drug sensitivity and resistance in tumor cells and other cultured cell types. These tools significantly improve upon conventional methodologies for dose-response studies by enabling researchers to account for the confounding effect of differences in cellular growth rate and to differentiate the cytotoxic and cytostatic effects of drug treatments across dose and time.
The topics covered will include: (1) automation technology for high-throughput screening; (2) experimental design strategies to ensure reproducibility and avoid common pitfalls in cultured cell dose-response studies; and (3) the theory and analytical implementation of improved dose-response metrics (PMID: 27135972 and the LINCS GR Calculator).
This lecture is open to the public. Interested graduate students are encouraged to register for the associated nano course: Assay automation and quantitation – from benchtop to HTS.