Illumina Synthetic Long Read data collected

December 30, 2014

We have now successfully generated long read data for two species using Illumina's new Synthetic Long Read kit. This kit size selects 8-10kb fragments, spreads them in low concentrations across 384 (barcoded) wells, and completes a Nextera-like tagmentation of molecules in each well. Short reads are generated with a HiSeq, and long reads are synthesized in BaseSpace.

One lane of sequencing per sample generated ~500Mb of long read data, with an N50 for longer reads (those ≥1500bp) of ~6500. Now on to exploring the best practices for using these data to increase our scaffold lengths during genome assembly...