Publications

2020
Audrey S. Vanhove, Bat-Erdene Jugder, Daniela Barraza, and Paula I. Watnick. 5/19/2020. “Methionine Availability in the Arthropod Intestine Is Elucidated through Identification of Vibrio cholerae Methionine Acquisition Systems.” Applied and Environmental Microbiology, 86, 11, Pp. e00371-20. Publisher's VersionAbstract
While only a subset of Vibrio cholerae strains are human diarrheal pathogens, all are aquatic organisms. In this environment, they often persist in close association with arthropods. In the intestinal lumen of the model arthropod Drosophila melanogaster, methionine and methionine sulfoxide decrease susceptibility to V. cholerae infection. In addition to its structural role in proteins, methionine participates in the methionine cycle, which carries out synthetic and regulatory methylation reactions. It is, therefore, essential for the growth of both animals and bacteria. Methionine is scarce in some environments, and the facile conversion of free methionine to methionine sulfoxide in oxidizing environments interferes with its utilization. To ensure an adequate supply of methionine, the genomes of most organisms encode multiple high-affinity uptake pathways for methionine as well as multiple methionine sulfoxide reductases, which reduce free and protein-associated methionine sulfoxide to methionine. To explore the role of methionine uptake and reduction in V. cholerae colonization of the arthropod intestine, we mutagenized the two high-affinity methionine transporters and five methionine sulfoxide reductases encoded in the V. cholerae genome. We show that MsrC is the sole methionine sulfoxide reductase active on free methionine sulfoxide. Furthermore, in the absence of methionine synthesis, high-affinity methionine uptake but not reduction is essential for V. cholerae colonization of the Drosophila intestine. These findings allow us to place a lower limit of 0.05 mM and an upper limit of 0.5 mM on the methionine concentration in the Drosophila intestine.
Bat-Erdene Jugder and Paula I. Watnick. 2/12/2020. “Vibrio cholerae Sheds Its Coat to Make Itself Comfortable in the Gut .” Cell Host & Microbe, 27, 2, Pp. 161-163. Publisher's Version
Paula I. Watnick and Bat-Erdene Jugder. 2/2020. “Microbial Control of Intestinal Homeostasis via Enteroendocrine Cell Innate Immune Signaling.” Trends in Microbiology, 28, 2, Pp. 141-149. Publisher's VersionAbstract
A community of commensal microbes, known as the intestinal microbiota, resides within the gastrointestinal tract of animals and plays a role in maintenance of host metabolic homeostasis and resistance to pathogen invasion. Enteroendocrine cells, which are relatively rare in the intestinal epithelium, have evolved to sense and respond to these commensal microbes. Specifically, they express G-protein-coupled receptors and functional innate immune signaling pathways that recognize products of microbial metabolism and microbe-associated molecular patterns, respectively. Here we review recent evidence from Drosophila melanogaster that microbial cues recruit antimicrobial, mechanical, and metabolic branches of the enteroendocrine innate immune system and argue that this response may play a role not only in maintaining host metabolic homeostasis but also in intestinal resistance to invasion by bacterial, viral, and parasitic pathogens.
2019
Nady Braidy, Martin Zarka, Bat-Erdene Jugder, Jeffrey Welch, Tharusha Jayasena, Daniel K. Y. Chan, Perminder Sachdev, and Wallace Bridge. 8/8/2019. “The Precursor to Glutathione (GSH), γ-Glutamylcysteine (GGC), Can Ameliorate Oxidative Damage and Neuroinflammation Induced by Aβ40 Oligomers in Human Astrocytes.” Frontiers in Aging Neuroscience, 11, 177. Publisher's Version
2018
Bat-Erdene Jugder, Karl AP Payne, Karl Fisher, Susanne Bohl, Helene Lebhar, Mike Manefield, Matthew Lee, David Leys, and Christopher P Marquis. 1/2018. “Heterologous production and purification of a functional chloroform reductive dehalogenase.” ACS chemical biology, 13, 3, Pp. 548-552. Publisher's Version
2017
Bat‐Erdene Jugder, Susanne Bohl, Helene Lebhar, Robert D Healey, Mike Manefield, Christopher P Marquis, and Matthew Lee. 11/2017. “A bacterial chloroform reductive dehalogenase: purification and biochemical characterization.” Microbial biotechnology, 10, 6, Pp. 1640-1648. Publisher's Version
Nady Braidy, Anne Poljak, Chris Marjo, Helen Rutlidge, Anne Rich, Bat-Erdene Jugder, Tharusha Jayasena, Nibaldo C Inestrosa, and Perminder S Sachdev. 3/29/2017. “Identification of Cerebral Metal Ion Imbalance in the Brain of Aging Octodon degus.” Frontiers in aging neuroscience, 9, 66. Publisher's Version
2016
Nady Braidy, Helene Rossez, Chai K Lim, Bat-Erdene Jugder, Bruce J Brew, and Gilles J Guillemin. 11/1/2016. “Characterization of the kynurenine pathway in CD8+ human primary monocyte-derived dendritic cells.” Neurotoxicity research, 30, 4, Pp. 620-632. Publisher's Version
Bat‐Erdene Jugder, Haluk Ertan, Yie Kuan Wong, Nady Braidy, Michael Manefield, Christopher P Marquis, and Matthew Lee. 10/1/2016. “Genomic, transcriptomic and proteomic analyses of Dehalobacter UNSWDHB in response to chloroform.” Environmental microbiology reports, 8, 5, Pp. 814-824. Publisher's Version
Bat-Erdene Jugder, Jeffrey Welch, Nady Braidy, and Christopher P Marquis. 7/26/2016. “Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein).” PeerJ, 4, Pp. e2269. Publisher's Version
Nady Braidy, Bat-Erdene Jugder, Anne Poljak, Tharusha Jayasena, Hussein Mansour, Seyed Mohammad Nabavi, Perminder Sachdev, and Ross Grant. 7/1/2016. “Resveratrol as a potential therapeutic candidate for the treatment and management of Alzheimer’s disease.” Current topics in medicinal chemistry, 16, 17, Pp. 1951-1960. Publisher's Version
Bat-Erdene Jugder, Helene Lebhar, Kondo-Francois Aguey-Zinsou, and Christopher P Marquis. 3/22/2016. “Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications.” MethodsX, 3, Pp. 242-250. Publisher's Version
Bat-Erdene Jugder, Haluk Ertan, Susanne Bohl, Matthew Lee, Christopher P Marquis, and Michael Manefield. 3/1/2016. “Organohalide respiring bacteria and reductive dehalogenases: key tools in organohalide bioremediation.” Frontiers in microbiology, 7, 249. Publisher's Version
2015
Bat-Erdene Jugder, Haluk Ertan, Matthew Lee, Michael Manefield, and Christopher P Marquis. 10/1/2015. “Reductive dehalogenases come of age in biological destruction of organohalides.” Trends in biotechnology, 33, 10, Pp. 595-610. Publisher's Version
Bat-Erdene Jugder, Zhiliang Chen, Darren Tan Tek Ping, Helene Lebhar, Jeffrey Welch, and Christopher P Marquis. 3/25/2015. “An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture.” Microbial cell factories, 14, 1, Pp. 42. Publisher's Version
2013
Bat-Erdene Jugder, Jeffrey Welch, Kondo-Francois Aguey-Zinsou, and Christopher P Marquis. 1/28/2013. “Fundamentals and electrochemical applications of [Ni–Fe]-uptake hydrogenases.” RSC Advances, 3, 22, Pp. 8142-8159. Publisher's Version