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Non-invasive techniques for analyzing and visualizing a wide range of systems, from nano-sized devices up to the human organs, are critical in research, industry, and healthcare. The course is designed as an introduction for students who want to gain both hands on training as well as an introduction to the physics and image reconstruction techniques involved in generating images. The course will introduce the fundamentals of the major imaging modalities including, but not limited to: electron microscopy, optical microscopy, x-ray computed tomography, ultrasound, MRI, and nuclear imaging, as well as an overview of in vivo and molecular imaging. Through current literature we will explore the applications of these imaging systems in bioengineering, electrical engineering, and clinical research. The fundamentals of each imaging system will coincide with examples of studies from the current literature and a laboratory component where students can learn how to collect images from many of these imaging systems. It is also useful for students in life sciences and engineering disciplines who may need to use imaging to assess their products, devices, or organisms.