Dr Marie Mortreux obtained her B.Sc (2010) and M.Sc (2012) in genetics and life sciences, along with a European Magistere of Genetics (2012), which were combined to laboratory work in l'Universita Degli Studi di Padova, Italy , at the University of California San Francisco, USA, and at Université Paris Diderot Paris VII in Paris, France. Her majors included Genetics, Genomics, Neurosciences, Physiology and Physiopathology. She then received her Ph.D. in physiopathology/cell biology in Paris in April 2016.
In the Argenton laboratory in Padua, Italy, Dr Mortreux helped develop several transgenic reporters for Sonic HedgeHog in the zebrafish. She purified plasmids, injected embryos, screened fish for accurate reporting, and identified founders to maintain the transgenic lines. The main skills used in this study were in vitro work, plasmid purification, electrophoresis, IHC, embryonic injection, animal husbandry and screening, and confocal microscopy.
In the Marcucio laboratory in San Francisco, CA, Dr Mortreux used WT and DMDmdx mice (model of Duchenne Muscular Dystrophy) to study the impact of muscle trauma and fracture in bone regeneration. The main skills used in this study were surgery (muscle trauma, 3 point fractures, both stabilized and un-stabilized), X-ray, post-surgical monitoring, microtome, sample processing (decalcification, paraffin embedding, histology staining), histomorphometry and stereology.
In the Magnan laboratory in Paris, France, Dr Mortreux investigated the role of Prokineticin-2 in the control of food intake and energy homeostasis, establishing if it was a good candidate to treat obesity and type 2 diabetes. Dr Mortreux engineered shRNAs that were tested in vitro before being included in lentiviruses. Recombinant protein was injected using stereotactic surgery either accutely (recombinant protein, single delivery), or chronically (JetPei vector, osmotic pumps). Dr Mortreux focuses on several murine models, lean mice, DIO mice (Diet-induced obesity by high fat diet), and Ob/Ob mice. Main skills included in vitro work, animal handling and husbandry, food preference test, CLAMS, echoMRI, ITT, OGTT, serology, cryostat sectioning, IHC, enzymatic assays, RTqPCR, WB, behavioral testing, laparotomy, stereotactic surgery, catheterization.
During her PhD, Dr Mortreux also teached at the university for undergrad students, using rats. She taught physiology (kidneys, reproductive physiology, endocrinology) and scientific methods for 2 years both in classes and during wet lab classes.
Dr Mortreux joinged the Rutkove laboratory at Harvard Medical School - BIDMC as a postdoctoral researcher, to focus on a NASA-funded project aiming to develop a model of partial gravity using rats. She focused on establishing the longitudinal time course of musculoskeletal alterations in response to reductions in mechanical loading. Soon after, she investigated the potential effects of a trip to Mars on the hindlimbs muscles, and assessed if Resveratrol, a known anti-diabetic polyphenol associated to many cellular pathways, could help preserve astronauts health. Her work has since been published and was featured at many conferences. She is featured in numerous international press articles.
In parallel, Dr Mortreux also uses electrical impedance myography and participates in research aiming at developing EIM to detect histopathological changes within the muscle, either in case of disuse, inflammation, or linked to a neuromuscular disorder. She recently refined EIM techniques to obtain in vivo estimation of muscle quality in animals exposed to an analogue of partial gravity of microgravity.
Dr Mortreux became an Instructor of Neurology in July 2019 and is now collaborating with several teams within the US, helping them to develop the partial gravity analog model and investigate other systems of interests, while pursuing her work in the Rutkove laboratory.
In January 2019 with 3 co-founders, Dr Mortreux created the BIDMC PostDoctoral Association. She is a board member, Chair of the Advocacy Committee and a member of the Career Development Committee, and is committed to improving the lives and work environments for the BIDMC Postdocs.
Dr Mortreux has joined the project "Skype a Scientist" in 2019, with whom she is giving classes remotely to promote youth engagement to STEM and a greater access to science.