Publications

2016
MA Liénard and C Löfstedt. 10/25/2016. “Small ermine moths : Role of sex pheromones in reproductive isolation and speciation.” In Pheromone Communication in Moths: Evolution, Behavior and Application, Allison, JD and Cardé RT (eds), Pp. pp. 211-224. Berkeley : University of California Press. (Invited Book Chapter). lienard_lofstedt_2016_9780520278561_-_13_ch13.pdf
MA Liénard, LO Araripe, and DL Hartl. 7/29/2016. “Neighboring Genes for DNA-Binding Proteins Rescue Male Sterility in Drosophila Hybrids.” Proceedings of the National Academy of Sciences, USA, 113, Pp. E4200-4207. pnas-2016-lienard-1608337113.pdf
2014
MA Liénard, H-L Wang, J-M Lassance, and C Löfstedt. 2014. “Sex pheromone biosynthetic pathways are conserved between moths and the butterfly Bicyclus anynana.” Nature Communications, 5:3957, Pp. DOI: 10.1038/ncomms4957. Publisher's Version Lienard_et_al_2014_ncomms4957.pdf
2013
J-M Lassance, MA Liénard, B Antony, S Qian, T Fujii, J Tabata, Y Ishikawa, and C Löfstedt. 2013. “Functional consequences of sequence variation in the pheromone biosynthetic gene pgFAR for Ostrinia moths.” Proceedings of the National Academy of Sciences, USA, 110, Pp. 3967-3972. Publisher's VersionAbstract

Pheromones are central to the mating systems of a wide range of organisms, and reproductive isolation between closely related species is often achieved by subtle differences in pheromone composition. In insects and moths in particular, the use of structurally similar components in different blend ratios is usually sufficient to impede gene flow between taxa. To date, the genetic changes associated with variation and divergence in pheromone signals remain largely unknown. Using the emerging model system Ostrinia, we show the functional consequences of mutations in the protein-coding region of the pheromone biosynthetic fatty-acyl reductase gene pgFAR. Heterologous expression confirmed that pgFAR orthologs encode enzymes exhibiting different substrate specificities that are the direct consequences of extensive nonsynonymous substitutions. When taking natural ratios of pheromone precursors into account, our data reveal that pgFAR substrate preference provides a good explanation of how species-specific ratios of pheromone components are obtained among Ostrinia species. Moreover, our data indicate that positive selection may have promoted the observed accumulation of nonsynonymous amino acid substitutions. Site-directed mutagenesis experiments substantiate the idea that amino acid polymorphisms underlie subtle or drastic changes in pgFAR substrate preference. Altogether, this study identifies the reduction step as a potential source of variation in pheromone signals in the moth genus Ostrinia and suggests that selection acting on particular mutations provides a mechanism allowing pheromone reductases to evolve new functional properties that may contribute to variation in the composition of pheromone signals.

Lassance_Lienard_et_al_2013_PNAS-1208706110.pdf
ÅK Hagström, H-L Wang, MA Liénard, J-M Lassance, T Johansson, and C Löfstedt. 2013. “A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory.” Microbial Cell FactoriesMicrobial Cell Factories, 12:125. Publisher's Version Pheromone_brewery_2013.pdf
2012
AK Hagström, MA Liénard, AT Groot, E Hedenström, and C Löfstedt. 2012. “Semi–Selective Fatty Acyl Reductases from Four Heliothine Moths Influence the Specific Pheromone Composition.” PLoS ONE, 7(5). Publisher's Version Hagström_et_al_plos_one_2012.pdf
J Albre, MA Liénard, TA Sirey, S Schmidt, LK Tooman, C Carraher, DR Greenwood, C Löfstedt, and RD Newcomb. 2012. “Sex pheromone evolution is associated with differential regulation of the same desaturase gene in two genera of leafroller moths.” PLoS Genetics, 8(1), e1002489, Pp. DOI:10.1371/journal.pgen.1002489. Publisher's Version Albre_Lienard_et_al_PLoS_Genetics_2012.pdf
2011
B-J Ding, MA Liénard, H-L Wang, C-H Zhao, and C Löfstedt. 2011. “Terminal fatty-acyl-CoA desaturase involved in sex pheromone biosynthesis in the winter moth (Operophtera brumata).” Insect Biochemistry and Molecular Biology, 41, Pp. 715-722. Publisher's Version Ding_Lienard_et_al_2011.pdf
2010
J-M Lassance, AT Groot, MA Liénard, B Antony, C Borgwart, DG Heckel, and C Löfstedt. 2010. “Allelic variation in a fatty-acyl reductase gene causes divergence in moth sex pheromones.” Nature, 466, Pp. 486-489. Publisher's Version lassance_et_al_2010.pdf
MA Liénard, J-M Lassance, H-L Wang, C-H Zhao, J Piskur, T Johansson, and C Löfstedt. 2010. “Elucidation of the sex-pheromone biosynthesis producing 5,7-dodecadienes in Dendrolimus punctatus (Lepidoptera: Lasiocampidae) reveals ∆11- and ∆9-desaturases with unusual catalytic properties.” Insect Biochemistry and Molecular Biology, 40, Pp. 440-452. Publisher's Version Lienard_et_al_ibmb_2010.pdf
MA Liénard. 2010. “Evolution of mate signalling in moths: Biosynthetic gene families and diversification of female sex pheromones.” Biology department. Publisher's Version
MA Liénard, ÅK Hagström, J-M Lassance, and C Löfstedt. 2010. “Evolution of multi-component pheromone signals in small ermine moths involves a single fatty-acyl reductase gene.” Proceedings of the National Academy of Sciences, USA, 107, Pp. 10955-10960. Publisher's Version Lienard_et_al_pnas_2010.pdf
MA Liénard and C Löfstedt. 2010. “Functional flexibility as a prelude to signal diversity? Role of a fatty acyl reductase in moth pheromone evolution.” Communicative and Integrative Biology, 3, Pp. 586-588 . Publisher's Version Lienard_and_Lofstedt_2010.pdf
H-L Wang*, MA Liénard*, C-H Zhao, C-Z Wang, and C Löfstedt. 2010. “Neofunctionalization in an ancestral insect desaturase lineage led to rare ∆6 pheromone signals in the Chinese tussah silkworm.” Insect Biochemistry and Molecular Biology, 40, Pp. 742-751. *equal contribution. Publisher's Version Wang*_Lienard*_et_al_2010_ibmb.pdf
2008
MA Liénard, M Strandh, E Hedenström, T Johansson, and C Löfstedt. 2008. “Key biosynthetic gene subfamily recruited for pheromone production prior to the extensive radiation of Lepidoptera.” BMC Evolutionary Biology, 8:270, Pp. doi:10.1186/1471-2148-8-270. Publisher's Version Lienard_et_al_2008_bmc.pdf
2006
MA Liénard, J-M Lassance, I Paulmier, J-F Picimbon, and C Löfstedt. 2006. “Differential expression of cytochrome c oxidase subunit III gene in castes of the termite Reticulitermes santonensis.” Journal of Insect Physiology, 52, Pp. 551-557. Publisher's Version Lienard_et_al_2006.pdf