Ryan Dinkele, Sophia Gessner, Andrea McKerry, Bryan Leonard, Ronnett Seldon, Anastasia S. Koch, Carl Morrow, Melitta Gqada, Mireille Kamariza, Carolyn R. Bertozzi, Brian Smith, Courtney McLoud, Andrew Kamholz, Wayne Bryden, Charles Call, Gilla Kaplan, Valerie Mizrahi, Robin Wood, and Digby F. Warner. 2021. “Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis patient bioaerosols.” PlosPathogens, 2, 17, Pp. e1009262. Publisher's Version
Tingting Dai, Jinghang Xie, Qihua Zhu, Mireille Kamariza, Ke Jiang, Jianghong Rao, and Carolyn R Bertozzi. 8/2020. “A Fluorogenic Trehalose Probe for Tracking Phagocytosed Mycobacterium tuberculosis.” Journal of the American Chemical Society. Publisher's Version
William E. Allen, Han Altae-Tran, James Briggs, Xin Jin, Glen McGee, Andy Shi, Rumya Raghavan, Mireille Kamariza, Nicole Nova, Albert Pereta, Chris Danford, Amine Kamel, Patrik Gothe, Evrhet Milam, Jean Aurambault, Thorben Primke, Weijie Li, Josh Inkenbrandt, Tuan Huynh, Evan Chen, Christina Lee, Michael Croatto, Helen Bentley, Wendy Lu, Robert Murray, Mark Travassos, Brent A. Coull, John Openshaw, Casey S. Greene, Ophir Shalem, Gary King, Ryan Probasco, David R. Cheng, Ben Silbermann, Feng Zhang, and Xihong Lin. 8/2020. “Population-scale longitudinal mapping of COVID-19 symptoms, behaviour and testing.” Nature Human Behaviour, 4, Pp. 972–982. Publisher's Version
Mireille Kamariza, Samantha G. L. Keyser, Ashley Utz, Benjamin D. Knapp, Green Ahn, C. J. Cambier, Teresia Chen, Kerwyn Casey Huang, and Carolyn R. Bertozzi. 5/30/2020. “Towards Mycobacterium tuberculosis detection at the point-of-care: a brighter solvatochromic probe permits the detection of mycobacteria within minutes.” BioRxiv. Publisher's Version
Jenny Stenger-Smith, Mireille Kamariza, Indranil Chakraborty, Ramatoulaye Ouattara, Carolyn R Bertozzi, and Pradip K Mascharak. 3/18/2020. “Enhanced Bactericidal Effects of Pyrazinamide Toward Mycobacterium smegmatis and Mycobacterium tuberculosis upon Conjugation to a {Au (I)-triphenylphosphine}+ Moiety.” ACS omega, 5, 12, Pp. 6826-6833. Publisher's Version
Benjamin James Patterson, Ryan Dinkele, Sophia Gessner, Carl Morrow, Mireille Kamariza, Carolyn R Bertozzi, Andrew Kamholz, Wayne Bryden, Charles Call, Digby F Warner, and Robin Wood. 1/1/2020. “Sensitivity Optimisation of Tuberculosis Bioaerosol Sampling.” MedRxiv. Publisher's Version
Carolyn R Bertozzi, Mireille Kamariza, and Peyton Shieh. 6/6/2019. “Methods for Detecting Mycobacteria with Solvatochromic Dye Conjugates.” United States of America 16321815. Publisher's Version
Ryan Dinkele, Sophia Gessner, Anastasia S. Koch, Carl Morrow, Melitta Gqada, Mireille Kamariza, Carolyn R. Bertozzi, Brian Smith, Courtney McLoud, Andrew Kamholz, Wayne Bryden, Charles Call, Valerie Mizrahi, and Digby F. Warner. 2019. “Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis bioaerosols.” BioRxiv. Publisher's Version
Mireille Kamariza, Peyton Shieh, Christopher S. Ealand, Julian S. Peters, Brian Chu, Frances P. Rodriguez-Rivera, Mohammed R. Babu Sait, William V. Treuren, Neil Martinson, Rainer Kalscheuer, Bavesh D. Kana, and Carolyn R. Bertozzi. 2/28/2018. “Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe.” Science Translational Medicine, 10, 430. Publisher's VersionAbstract
Tuberculosis (TB) is the leading cause of death from an infectious bacterial disease. Poor diagnostic tools to detect active disease plague TB control programs and affect patient care. Accurate detection of live Mycobacterium tuberculosis (Mtb), the causative agent of TB, could improve TB diagnosis and patient treatment. We report that mycobacteria and other corynebacteria can be specifically detected with a fluorogenic trehalose analog. We designed a 4-N,N-dimethylamino-1,8-naphthalimide–conjugated trehalose (DMN-Tre) probe that undergoes >700-fold increase in fluorescence intensity when transitioned from aqueous to hydrophobic environments. This enhancement occurs upon metabolic conversion of DMN-Tre to trehalose monomycolate and incorporation into the mycomembrane of Actinobacteria. DMN-Tre labeling enabled the rapid, no-wash visualization of mycobacterial and corynebacterial species without nonspecific labeling of Gram-positive or Gram-negative bacteria. DMN-Tre labeling was detected within minutes and was inhibited by heat killing of mycobacteria. Furthermore, DMN-Tre labeling was reduced by treatment with TB drugs, unlike the clinically used auramine stain. Lastly, DMN-Tre labeled Mtb in TB-positive human sputum samples comparably to auramine staining, suggesting that this operationally simple method may be deployable for TB diagnosis.
Mireille Kamariza, Peyton Shieh, and Carolyn R. Bertozzi. 11/27/2017. “Imaging Mycobacterial Trehalose Glycolipids.” Methods in Enzymology, 598, Pp. 355-369. Publisher's VersionAbstract
Cell surface trehalose mycolates are important modulators of mycobacterial pathogenesis and host immune response. We discuss the use of fluorescent and fluorogenic trehalose probes for the detection of the mycobacterial trehalose glycolipids. These probes enable real-time imaging of trehalose mycolate biosynthesis and mycomembrane dynamics in the laboratory as well as in clinical settings for the detection of mycobacteria in patient samples.