Ever since my lab posted the SCoPE-MS preprint, I have been repeatedly asked about the future potential and the cost of quantifying proteins by high-throughput mass-spectrometry in single cells. I will summarize a few thoughts that hopefully will be helpful and will reduce email traffic.
Why quantify proteins and PTMs in single cells?
Single-cell RNA-seq has made great strides and become widely available and preferred method for high-throughput single-cell measurements. That is great! These...
slavovLabThe monthly video of the lecture series 𝑺𝒕𝒂𝒕𝒊𝒔𝒕𝒊𝒄𝒔 𝒇𝒐𝒓 𝒑𝒓𝒐𝒕𝒆𝒐𝒎𝒊𝒄𝒔 is focused on non-ignorable missing data.
Ignoring non-ignorable missingness leads to survivor bias & other artifacts t.co/v4rlCT2lust.co/HHmH8qVBbw