Ever since my lab posted the SCoPE-MS preprint, I have been repeatedly asked about the future potential and the cost of quantifying proteins by high-throughput mass-spectrometry in single cells. I will summarize a few thoughts that hopefully will be helpful and will reduce email traffic.

Why quantify proteins and PTMs in single cells?

Single-cell RNA-seq has made great strides and become widely available and preferred method for high-throughput single-cell measurements. That is great! These...