Nature methods published recently 2 features highlighting single-cell proteomics by mass-spectrometry featuring contributions from the Slavov Lab:
A dream of single-cell proteomics, Nature Methods volume 16, pages 809–812(2019), doi: 10.1038/s41592-019-0540-6
Voices in methods development, Nature Methods volume 16, pages 945–951(2019), doi: 10.1038/s41592-019-0585-6
Recently, mass-spectrometry methods have increased the specificity and throughput of quantifying proteins in single mammalian cells: we can now quantify thousands of proteins across hundreds of single cells. I am confident that soon we will extend these methods to quantifying metabolites, post-translational modifications, and the dynamics and spatial distributions of proteins and their complexes. Ultimately, the accuracy, completeness and throughput of these measurements will provide data for transitioning from descriptive classification of single cells to quantitative models of regulatory protein interactions. I believe these data and models will enable systematic inference of direct causal mechanisms that underpin biological functions.