Contrary to the textbook model, recent measurements demonstrated unexpected diversity in ribosomal composition that likely enables specialized translational functions. Methods based on liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) enable direct quantification of ribosomal proteins with high specificity, accuracy, and throughput. LC-MS/MS can be ‘top-down’, analyzing intact proteins, or more commonly ‘bottom-up’, where proteins are digested to peptides prior to analysis. Changes to rRNA can be examined using either LC-MS/MS or sequencing-based approaches. The regulation of protein synthesis by specialized ribosomes can be examined by multiple methods. These include the popular ‘Ribo-Seq’ method for analyzing ribosome density on a given mRNA, as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor protein synthesis and degradation.
slavovLab@galos_gann Another problem with such comments is that they can be obfuscated in convolute technical jargon, making them rather hard for professional editors to evaluate. They just make everybody's life harder for no good reason.
slavovLab@rmounce Perhaps the the questions over the credibility of #OA journals are raised my parties having selfish interests to undermine the march of #OA and preserve as long as possible the system that nourishes them.
slavovLab@galos_gann The point of peer review is constructive feedback, not highly-technical comments intended to confuse.
I raise this publicly because I think it's poisonous for the community. It's counterproductive. It's sad. It's pathetic.