Contrary to the textbook model, recent measurements demonstrated unexpected diversity in ribosomal composition that likely enables specialized translational functions. Methods based on liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) enable direct quantification of ribosomal proteins with high specificity, accuracy, and throughput. LC-MS/MS can be ‘top-down’, analyzing intact proteins, or more commonly ‘bottom-up’, where proteins are digested to peptides prior to analysis. Changes to rRNA can be examined using either LC-MS/MS or sequencing-based approaches. The regulation of protein synthesis by specialized ribosomes can be examined by multiple methods. These include the popular ‘Ribo-Seq’ method for analyzing ribosome density on a given mRNA, as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor protein synthesis and degradation.
slavovLabThe French must have had very inebriated time all the time in the 1920s, with 184 liters of wine / person per year. That is probably 1-2 bottles of wine per adult per day ... every day, unless children actively helped with that alcohol feast!
@MaxCRoser, that's hard to imagine. t.co/WWsXN2cau0