The performance of ultrasensitive liquid chromatography and tandem mass spectrometry (LC-MS/MS) methods, such as single-cell proteomics by mass spectrometry (SCoPE-MS), depends on multiple interdependent parameters. This interdependence makes it challenging to specifically pinpoint the sources of problems in the LC-MS/MS methods and approaches for resolving them. For example, a low signal at the MS2 level can be due to poor LC separation, ionization, apex targeting, ion transfer, or ion detection. We sought to specifically diagnose such problems by interactively visualizing data from all levels of bottom-up LC-MS/MS analysis. Many software packages, such as MaxQuant, already provide such data, and we developed an open source platform for their interactive visualization and analysis: Data-driven Optimization of MS (DO-MS). We found that in many cases DO-MS not only specifically diagnosed LC-MS/MS problems but also enabled us to rationally optimize them. For example, by using DO-MS to optimize the sampling of the elution peak apexes, we increased ion accumulation times and apex sampling, which resulted in a 370% more efficient delivery of ions for MS2 analysis. DO-MS is easy to install and use, and its GUI allows for interactive data subsetting and high-quality figure generation. The modular design of DO-MS facilitates customization and expansion. DO-MS v1.0.8 is available for download from GitHub: https://github.com/SlavovLab/DO-MS. Additional documentation is available at https://do-ms.slavovlab.net.
slavovLabOn Thursday, I will visit @harvardmed and will present, among other projects, my favorite experiment t.co/m25JbmMq3j I have not presented it in awhile, and I am delighted to be asked to talk about it.
Remembering that Christmas makes me smile t.co/wix4zQM8sG
slavovLab@bornspectator42 One factor is that MIT undergrads in life sciences tend to have stronger math skills than the PhD students, in part because MIT requires all undergraduates to take basic math classes.
I personally think all undergraduates majoring in science should be required to take basic math
slavovLabRecently two MIT professors told me that their graduate classes are simplified (in their words "watered down") versions of their undergraduate classes... which is what David Botstein used to say for his genetics classes at MIT long time ago.