%0 Journal Article %J Nat Genet %D 2019 %T Landscape of B cell immunity and related immune evasion in human cancers %A Xihao Hu %A Zhang, Jian %A Wang, Jin %A Jingxin Fu %A Li, Taiwen %A Zheng, Xiaoqi %A Wang, Binbin %A Shengqing Gu %A Jiang, Peng %A Fan, Jingyu %A Ying, Xiaomin %A Zhang, Jing %A Carroll, Michael C %A Wucherpfennig, Kai W %A Hacohen, Nir %A Fan Zhang %A Zhang, Peng %A Liu, Jun S %A Li, Bo %A X. Shirley Liu %X Tumor-infiltrating B cells are an important component in the microenvironment but have unclear anti-tumor effects. We enhanced our previous computational algorithm TRUST to extract the B cell immunoglobulin hypervariable regions from bulk tumor RNA-sequencing data. TRUST assembled more than 30 million complementarity-determining region 3 sequences of the B cell heavy chain (IgH) from The Cancer Genome Atlas. Widespread B cell clonal expansions and immunoglobulin subclass switch events were observed in diverse human cancers. Prevalent somatic copy number alterations in the MICA and MICB genes related to antibody-dependent cell-mediated cytotoxicity were identified in tumors with elevated B cell activity. The IgG3-1 subclass switch interacts with B cell-receptor affinity maturation and defects in the antibody-dependent cell-mediated cytotoxicity pathway. Comprehensive pancancer analyses of tumor-infiltrating B cell-receptor repertoires identified novel tumor immune evasion mechanisms through genetic alterations. The IgH sequences identified here are potentially useful resources for future development of immunotherapies. %B Nat Genet %V 51 %P 560-567 %8 2019 Mar %G eng %N 3 %1 http://www.ncbi.nlm.nih.gov/pubmed/30742113?dopt=Abstract %R 10.1038/s41588-018-0339-x