@article {611587,
	title = {Reemergence and amplification of tuberculosis in the Canadian arctic},
	journal = {J Infect Dis},
	volume = {211},
	number = {12},
	year = {2015},
	month = {2015 Jun 15},
	pages = {1905-14},
	abstract = {BACKGROUND: Between November 2011 and November 2012, a Canadian village of 933 persons had 50 culture-positive cases of tuberculosis, with 49 sharing the same genotype.

METHODS: We performed Illumina-based whole-genome sequencing on Mycobacterium tuberculosis isolates from this village, during and before the outbreak. Phylogenetic trees were generated using the maximum likelihood method.

RESULTS: Three distinct genotypes were identified. Strain I (n = 7) was isolated in 1991-1996. Strain II (n = 8) was isolated in 1996-2004. Strain III (n = 62) first appeared in 2007 and did not arise from strain I or II. Within strain III, there were 3 related but distinct clusters: IIIA, IIIB, and IIIC. Between 2007 and 2010, cluster IIIA predominated (11 of 22 vs 2 of 40; P < .001), whereas in 2011-2012 clusters IIIB (n = 18) and IIIC (n = 20) predominated over cluster IIIA (n = 11). Combined evolutionary and epidemiologic analysis of strain III cases revealed that the outbreak in 2011-2012 was the result of >=6 temporally staggered events, spanning from 1 reactivation case to a point-source outbreak of 20 cases.

CONCLUSIONS: After the disappearance of 2 strains of M. tuberculosis in this village, its reemergence in 2007 was followed by an epidemiologic amplification, affecting >5\% of the population.},
	keywords = {Adolescent, Adult, Arctic Regions, Canada, Communicable Diseases, Emerging, Disease Outbreaks, Female, Genome, Bacterial, Genotype, Humans, Male, Mycobacterium tuberculosis, phylogeny, Sequence Analysis, DNA, Tuberculosis, Young Adult},
	issn = {1537-6613},
	doi = {10.1093/infdis/jiv011},
	author = {Lee, Robyn S and Radomski, Nicolas and Proulx, Jean-Francois and Manry, Jeremy and McIntosh, Fiona and Desjardins, Francine and Soualhine, Hafid and Domenech, Pilar and Reed, Michael B and Menzies, Dick and Behr, Marcel A}
}
