@article {611591,
	title = {Population genomics of Mycobacterium tuberculosis in the Inuit},
	journal = {Proc Natl Acad Sci U S A},
	volume = {112},
	number = {44},
	year = {2015},
	month = {2015 Nov 03},
	pages = {13609-14},
	abstract = {Nunavik, Qu{\'e}bec suffers from epidemic tuberculosis (TB), with an incidence 50-fold higher than the Canadian average. Molecular studies in this region have documented limited bacterial genetic diversity among Mycobacterium tuberculosis isolates, consistent with a founder strain and/or ongoing spread. We have used whole-genome sequencing on 163 M. tuberculosis isolates from 11 geographically isolated villages to provide a high-resolution portrait of bacterial genetic diversity in this setting. All isolates were lineage 4 (Euro-American), with two sublineages present (major, n = 153; minor, n = 10). Among major sublineage isolates, there was a median of 46 pairwise single-nucleotide polymorphisms (SNPs), and the most recent common ancestor (MRCA) was in the early 20th century. Pairs of isolates within a village had significantly fewer SNPs than pairs from different villages (median: 6 vs. 47, P < 0.00005), indicating that most transmission occurs within villages. There was an excess of nonsynonymous SNPs after the diversification of M. tuberculosis within Nunavik: The ratio of nonsynonymous to synonymous substitution rates (dN/dS) was 0.534 before the MRCA but 0.777 subsequently (P = 0.010). Nonsynonymous SNPs were detected across all gene categories, arguing against positive selection and toward genetic drift with relaxation of purifying selection. Supporting the latter possibility, 28 genes were partially or completely deleted since the MRCA, including genes previously reported to be essential for M. tuberculosis growth. Our findings indicate that the epidemiologic success of M. tuberculosis in this region is more likely due to an environment conducive to TB transmission than a particularly well-adapted strain. },
	keywords = {Genes, Bacterial, Genetics, Population, Humans, Inuits, Mycobacterium tuberculosis, Polymorphism, Single Nucleotide, Quebec, Selection, Genetic, Tuberculosis},
	issn = {1091-6490},
	doi = {10.1073/pnas.1507071112},
	author = {Lee, Robyn S and Radomski, Nicolas and Proulx, Jean-Francois and Levade, Ines and Shapiro, B Jesse and McIntosh, Fiona and Soualhine, Hafid and Menzies, Dick and Behr, Marcel A}
}
