@article {669478,
	title = {Mitochondrial genomes of the Dorcus velutinus complex (Coleoptera: Lucanidae) with the large intergenic spacer showing unique short sequence repeats and their implications for systematics},
	journal = {Journal of Asia-Pacific Entomology},
	volume = {24},
	number = {1},
	year = {2021},
	pages = {493-501},
	abstract = {Mitochondrial genomes of the three lucanid species in the\&nbsp;Dorcus velutinus\&nbsp;complex {\textendash}\&nbsp;Dorcus velutinus\&nbsp;Thomson,\&nbsp;D. ursulus\&nbsp;Arrow and\&nbsp;D. tenuihirsutus\&nbsp;Kim and Kim\&nbsp;{\textendash}\&nbsp;were assembled and analyzed through next generation sequencing. The mitogenome sequences were used to infer phylogenetic relationships among\&nbsp;Dorcus\&nbsp;species. Our analyses revealed that the newly sequenced mitogenomes are comparable in their size, content, and gene arrangement to other lucanid mitogenomes reported to date. However, we confirmed the presence of a large intergenic spacer (IGS) between\&nbsp;trnS(UCN)\&nbsp;and ND1 genes, whose length varied from 170\&nbsp;bp (in\&nbsp;D. tenuihirsutus) to 193\&nbsp;bp (in\&nbsp;D. ursulus\&nbsp;and\&nbsp;D. velutinus). Within this IGS region, a short sequence fragment (TACTAAATT) was found uniquely across the three species of\&nbsp;Dorcus velutinus\&nbsp;complex. Our phylogenetic analyses show that the\&nbsp;D. velutinus\&nbsp;complex constitutes a distinct clade with a significant divergence from other species of the genus\&nbsp;Dorcus\&nbsp;sensu stricto. Furthermore, we reaffirm the validity of\&nbsp;D. tenuihirsutus {\textendash} a species originally described from Korea {\textendash} as a distinct species, though the taxonomic status of\&nbsp;D. ursulus\&nbsp;remains to be studied further. Finally, we find the presence and location of large IGSs to be useful for studying evolutionary history and species delimitation in stag beetles.},
	url = {https://www.sciencedirect.com/science/article/abs/pii/S1226861520302661},
	author = {Chen, Y-J and S.Kim and Xia, W.}
}
