The following movies demonstrate some of the methods in our studies.
- chemotaxis.avi - This movie shows a few C. elegans navigate towards an attractive odor in the center of the movie.
- dropletassay_02.avi - This movie shows the microdroplet assay that quantifies olfactory preference based on the turning rate. Sharp turns resembles the shape of the Greek letter omega. Attractive odors suppress sharp turns and repulsive conditions increase turns. Turns are automatically counted by a computer software. For details, please refer to our paper Ha et al., Neuron 2010.
- worm_imaging_chip.avi - This movie shows the microfluidic device that we use to image neuronal activity in live animals that perform olfactory tasks. We can precisely control the delivery of the chemical stimuli to the animal by controlling the fluidic streams. The design of the chip was based on Chronis et al., Nat Methods 2007.
- RIA_axonal_activity.mov - This movie shows the compartmentalized axonal calcium signals in the interneurons RIA and the head movement of a transgenic animal that expresses GCaMP3 in RIA. You can see that calcium signals in the ventral and dorsal RIA axonal compartments in the nerve ring (nrV and nrD) correlate with ventral and dorsal head bending, respectively. Anterior is to the left and dorsal is up. For details, please refer to our paper Hendricks et al., Nature 2012.
- SMDV_SMDD_activity.mov - This movie shows GCaMP3 calcium signals in SMDV, SMDD motor neurons and head movement of a transgenic animal. You can see that the calcium signals in SMDV and SMDD correlate with ventral and dorsal head bending, respectively. Anterior is to the left and dorsal is up. For details, please refer to our paper Hendricks et al., Nature 2012.
- gait_quantification.avi - This movie shows the locomotion tracking of a wild-type animal (upper trace) and a mutant animal that has lost RIA compartmentalized axonal activity (lower trace). You can see that the mutant displays larger amplitude of headbending.